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The effect of PZQ on NTS was determined using the formula % Effect = 100 – (Average (Test) x 100 / Average (Control)) and LD 50 determined in GraphPad Prism

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[18] The disulfide bridge of gliotoxin is crucial to its toxicity, so it is theorized that the tailoring of enzymes to prevent the disulfide bridge closure by interfering with GliT or by catalyzing another reaction to block the sulfur residues may be beneficial in reducing the toxicity of those fungi